User:Allison K. Alix/Notebook/CHEM-581/2013/03/08

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(Objective)
(Description)
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-Measure the amount of dye absorbed
-Measure the amount of dye absorbed
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==Description==
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==Procedures==
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0.1 g of hydrogel (1 sample with lecithin and one without) in 5mL phosphate buffer
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1) Add 0.1 g of hydrogel (1 sample with lecithin and one without) in 5mL phosphate buffer
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sonicate for ~25 minutes
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2) Sonicate for ~25 minutes or until hydrogels have separated
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Add 5 μL of dye
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3) Add 5 μL of dye.
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4) Allow hydrogels to absorb dye overnight
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5) Calculate the amount of dye absorbed by the hydrogels by measuring the concentration left in solution
==Data==
==Data==

Revision as of 17:38, 26 March 2013

Measuring Dye Absorbance Main project page
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Objectives

-Suspend hydrogels back into solution

-Attach dye to hydrogels once they are in solution

-Measure the amount of dye absorbed

Procedures

1) Add 0.1 g of hydrogel (1 sample with lecithin and one without) in 5mL phosphate buffer

2) Sonicate for ~25 minutes or until hydrogels have separated

3) Add 5 μL of dye.

4) Allow hydrogels to absorb dye overnight

5) Calculate the amount of dye absorbed by the hydrogels by measuring the concentration left in solution

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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