User:Allison K. Alix/Notebook/CHEM-581/2013/03/08

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Description)
(Procedures)
Line 24: Line 24:
3) Add 5 μL of dye.
3) Add 5 μL of dye.
-
4) Allow hydrogels to absorb dye overnight
+
4) Allow hydrogels to absorb dye  
-
5) Calculate the amount of dye absorbed by the hydrogels by measuring the concentration left in solution
+
5) Measure the absorbance of the solution in 15 minute intervals (1mL aliquots)
 +
 
 +
6) Add 1 mL distilled water for every 1mL taken out of solution
==Data==
==Data==

Revision as of 16:45, 26 March 2013

Measuring Dye Absorbance Main project page
Previous entry      Next entry


Objectives

-Suspend hydrogels back into solution

-Attach dye to hydrogels once they are in solution

-Measure the amount of dye absorbed

Procedures

1) Add 0.1 g of hydrogel (1 sample with lecithin and one without) in 5mL phosphate buffer

2) Sonicate for ~25 minutes or until hydrogels have separated

3) Add 5 μL of dye.

4) Allow hydrogels to absorb dye

5) Measure the absorbance of the solution in 15 minute intervals (1mL aliquots)

6) Add 1 mL distilled water for every 1mL taken out of solution

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



Personal tools