User:Allison K. Alix/Notebook/CHEM-581/2013/03/08: Difference between revisions
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3) Add 5 μL of dye. | 3) Add 5 μL of dye. | ||
4) Allow hydrogels to absorb dye | 4) Allow hydrogels to absorb dye | ||
5) | 5) Measure the absorbance of the solution in 15 minute intervals (1mL aliquots) | ||
6) Add 1 mL distilled water for every 1mL taken out of solution | |||
==Data== | ==Data== |
Revision as of 14:45, 26 March 2013
Measuring Dye Absorbance | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Objectives-Suspend hydrogels back into solution -Attach dye to hydrogels once they are in solution -Measure the amount of dye absorbed Procedures1) Add 0.1 g of hydrogel (1 sample with lecithin and one without) in 5mL phosphate buffer 2) Sonicate for ~25 minutes or until hydrogels have separated 3) Add 5 μL of dye. 4) Allow hydrogels to absorb dye 5) Measure the absorbance of the solution in 15 minute intervals (1mL aliquots) 6) Add 1 mL distilled water for every 1mL taken out of solution Data
NotesThis area is for any observations or conclusions that you would like to note.
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