User:Allison K. Alix/Notebook/CHEM-581/2013/03/08: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 18: Line 18:


==Procedures==
==Procedures==
Part 1
1) Add 0.1 g of hydrogel (1 sample with lecithin and one without) in 5mL phosphate buffer
1) Add 0.1 g of hydrogel (1 sample with lecithin and one without) in 5mL phosphate buffer


Line 29: Line 32:


6) Add 1 mL distilled water for every 1mL taken out of solution
6) Add 1 mL distilled water for every 1mL taken out of solution
Part 2
1) Place 2mg of PVOH (w/ R6G, w/o lecithin) in 1.5mL of the following solutions:
  a) distilled water
  b) phosphate buffer
  c) 1M NaOH
  d) 1M HCl
  e) glutaraldehyde fixant solution (NaCl)
2) Allow hydrogels to stay in solution for 1 hour
3) Centrifuge for 5 minutes
4) Take absorbance measurements


==Data==
==Data==

Revision as of 14:49, 26 March 2013

Measuring Dye Absorbance <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


Objectives

-Suspend hydrogels back into solution

-Attach dye to hydrogels once they are in solution

-Measure the amount of dye absorbed

Procedures

Part 1

1) Add 0.1 g of hydrogel (1 sample with lecithin and one without) in 5mL phosphate buffer

2) Sonicate for ~25 minutes or until hydrogels have separated

3) Add 5 μL of dye.

4) Allow hydrogels to absorb dye

5) Measure the absorbance of each solution in 15 minute intervals (1mL aliquots)

6) Add 1 mL distilled water for every 1mL taken out of solution

Part 2

1) Place 2mg of PVOH (w/ R6G, w/o lecithin) in 1.5mL of the following solutions: 

  a) distilled water
  b) phosphate buffer
  c) 1M NaOH
  d) 1M HCl
  e) glutaraldehyde fixant solution (NaCl)

2) Allow hydrogels to stay in solution for 1 hour

3) Centrifuge for 5 minutes

4) Take absorbance measurements

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Allison_K._Alix/Notebook/CHEM-581/2013/03/08&oldid=686291"