User:Allison K. Alix/Notebook/CHEM-581/2013/03/08: Difference between revisions
| Line 17: | Line 17: | ||
-Measure the amount of dye absorbed | -Measure the amount of dye absorbed | ||
== | ==Procedures== | ||
0.1 g of hydrogel (1 sample with lecithin and one without) in 5mL phosphate buffer | 1) Add 0.1 g of hydrogel (1 sample with lecithin and one without) in 5mL phosphate buffer | ||
2) Sonicate for ~25 minutes or until hydrogels have separated | |||
Add 5 μL of dye | 3) Add 5 μL of dye. | ||
4) Allow hydrogels to absorb dye overnight | |||
5) Calculate the amount of dye absorbed by the hydrogels by measuring the concentration left in solution | |||
==Data== | ==Data== | ||
Revision as of 14:38, 26 March 2013
 Measuring Dye Absorbance
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
|
Objectives-Suspend hydrogels back into solution -Attach dye to hydrogels once they are in solution -Measure the amount of dye absorbed Procedures1) Add 0.1 g of hydrogel (1 sample with lecithin and one without) in 5mL phosphate buffer 2) Sonicate for ~25 minutes or until hydrogels have separated 3) Add 5 μL of dye. 4) Allow hydrogels to absorb dye overnight 5) Calculate the amount of dye absorbed by the hydrogels by measuring the concentration left in solution Data
NotesThis area is for any observations or conclusions that you would like to note.
| |
