User:Allison K. Alix/Notebook/CHEM-581/2013/03/08
|Measuring Dye Absorbance|| Main project page|
Previous entry Next entry
-Suspend hydrogels back into solution
-Attach dye to hydrogels once they are in solution
-Measure the amount of dye absorbed
1) Add 0.1 g of hydrogel (1 sample with lecithin and one without) in 5mL phosphate buffer
2) Sonicate for ~25 minutes or until hydrogels have separated
3) Add 5 μL of dye.
4) Allow hydrogels to absorb dye overnight
5) Calculate the amount of dye absorbed by the hydrogels by measuring the concentration left in solution
This area is for any observations or conclusions that you would like to note.