User:Allison K. Alix/Notebook/CHEM-581/2013/03/27

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Procedures)
(Procedures)
Line 17: Line 17:
==Procedures==
==Procedures==
-
Part 1:
+
Part 1: Finish preparing liposome coated hydrogels
1) Complete five freeze-thaw vortexes using liquid nitrogen on hydrogel-liposome solution(Freeze for 30 seconds or until frozen, thaw for 30 seconds or until thawed and vortex for 30 seconds. Repeat)
1) Complete five freeze-thaw vortexes using liquid nitrogen on hydrogel-liposome solution(Freeze for 30 seconds or until frozen, thaw for 30 seconds or until thawed and vortex for 30 seconds. Repeat)
Line 24: Line 24:
3) Resuspend in tris-HCl buffer
3) Resuspend in tris-HCl buffer
 +
 +
Part 2: Preparing a concentration gradient of R6G in hydrogels
 +
 +
1) Add the following volumes of 1mM R6G to 2mg PVOH hydrogels in 1.5mL phosphate buffer:
 +
 +
10μL, 15μL, 20μL, 25μL, 30μL, 35μL
 +
 +
2) Allow absorption of dye for 48 hours
==Data==
==Data==

Revision as of 09:48, 27 March 2013

Experimental Chemistry Main project page
Previous entry      Next entry


Objective

-Convert bound ULVs into a single bilayer coating the microgels

-Observe hydrogels containing dye under microscope

Procedures

Part 1: Finish preparing liposome coated hydrogels

1) Complete five freeze-thaw vortexes using liquid nitrogen on hydrogel-liposome solution(Freeze for 30 seconds or until frozen, thaw for 30 seconds or until thawed and vortex for 30 seconds. Repeat)

2) Remove unbound ULVs by centrifuging at 4000 rpm for 5 minutes

3) Resuspend in tris-HCl buffer

Part 2: Preparing a concentration gradient of R6G in hydrogels

1) Add the following volumes of 1mM R6G to 2mg PVOH hydrogels in 1.5mL phosphate buffer:

10μL, 15μL, 20μL, 25μL, 30μL, 35μL

2) Allow absorption of dye for 48 hours

Data

  • Add data and results here...

References

Lipogels: Single-Lipid-Bilayer-Enclosed Hydrogel Spheres Qasim Saleem,†,§,|| Baoxu Liu,§,‡,||Claudiu C. Gradinaru,*,§,‡ and Peter M. Macdonald


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



Personal tools