User:Allison K. Alix/Notebook/CHEM-581/2013/03/27: Difference between revisions
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==Procedures== | ==Procedures== | ||
Part 1: | Part 1: Finish preparing liposome coated hydrogels | ||
1) Complete five freeze-thaw vortexes using liquid nitrogen on hydrogel-liposome solution(Freeze for 30 seconds or until frozen, thaw for 30 seconds or until thawed and vortex for 30 seconds. Repeat) | 1) Complete five freeze-thaw vortexes using liquid nitrogen on hydrogel-liposome solution(Freeze for 30 seconds or until frozen, thaw for 30 seconds or until thawed and vortex for 30 seconds. Repeat) | ||
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3) Resuspend in tris-HCl buffer | 3) Resuspend in tris-HCl buffer | ||
Part 2: Preparing a concentration gradient of R6G in hydrogels | |||
1) Add the following volumes of 1mM R6G to 2mg PVOH hydrogels in 1.5mL phosphate buffer: | |||
10μL, 15μL, 20μL, 25μL, 30μL, 35μL | |||
2) Allow absorption of dye for 48 hours | |||
==Data== | ==Data== |
Revision as of 07:48, 27 March 2013
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Objective-Convert bound ULVs into a single bilayer coating the microgels -Observe hydrogels containing dye under microscope ProceduresPart 1: Finish preparing liposome coated hydrogels 1) Complete five freeze-thaw vortexes using liquid nitrogen on hydrogel-liposome solution(Freeze for 30 seconds or until frozen, thaw for 30 seconds or until thawed and vortex for 30 seconds. Repeat) 2) Remove unbound ULVs by centrifuging at 4000 rpm for 5 minutes 3) Resuspend in tris-HCl buffer Part 2: Preparing a concentration gradient of R6G in hydrogels 1) Add the following volumes of 1mM R6G to 2mg PVOH hydrogels in 1.5mL phosphate buffer: 10μL, 15μL, 20μL, 25μL, 30μL, 35μL 2) Allow absorption of dye for 48 hours Data
ReferencesLipogels: Single-Lipid-Bilayer-Enclosed Hydrogel Spheres Qasim Saleem,†,§,|| Baoxu Liu,§,‡,||Claudiu C. Gradinaru,*,§,‡ and Peter M. Macdonald
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