User:Allison K. Alix/Notebook/CHEM-581/2013/03/27: Difference between revisions
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-Observe hydrogels containing dye under microscope | -Observe hydrogels containing dye under microscope | ||
== | ==Procedures== | ||
Part 1: | |||
1) Complete five freeze-thaw vortexes using liquid nitrogen | |||
2) Remove unbound ULVs by centrifuging at 4000 rpm for 5 minutes | |||
3) Resuspend in tris-HCl buffer | |||
==Data== | ==Data== | ||
Revision as of 05:27, 27 March 2013
 Experimental Chemistry
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Objective-Convert bound ULVs into a single bilayer coating the microgels -Observe hydrogels containing dye under microscope ProceduresPart 1: 1) Complete five freeze-thaw vortexes using liquid nitrogen 2) Remove unbound ULVs by centrifuging at 4000 rpm for 5 minutes 3) Resuspend in tris-HCl buffer Data
ReferencesLipogels: Single-Lipid-Bilayer-Enclosed Hydrogel Spheres Qasim Saleem,†,§,|| Baoxu Liu,§,‡,||Claudiu C. Gradinaru,*,§,‡ and Peter M. Macdonald
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