User:Allison K. Alix/Notebook/CHEM-581/2013/03/29

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(Procedures)
Current revision (07:08, 3 April 2013) (view source)
(Procedures)
 
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Part 3: Prepare hydrogels with indicators
Part 3: Prepare hydrogels with indicators
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1) Measure out 3 0.5mg samples of PVOh hydrogels containing no lecithin and no dye
+
1) Measure out 3 0.5mg samples of PVOH hydrogels containing no lecithin and no dye
2) Dissolve each in 1.5mL phosphate buffer.
2) Dissolve each in 1.5mL phosphate buffer.

Current revision

Experimental Chemistry Main project page
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Objective

-Centrifuge concentration gradient solutions to observe absorbance in each

-Observe pH changes in crystal violet hydrogels with the addition of HCl or NaOH

Procedures

Part 1: Preparing Concentration Gradients for UV-Vis Measurements

1) Centrifuge 6 solutions containing varying amounts of R6G prepared on 03/27/2013 for 5 minutes at 11200rpm 3 times.

2) Make the following stock solutions to compare the supernatant absorbances to:

  • 10μL R6G in 1.5mL phosphate buffer
  • 15μL R6G in 1.5mL phosphate buffer
  • 20μL R6G in 1.5mL phosphate buffer
  • 25μL R6G in 1.5mL phosphate buffer
  • 30μL R6G in 1.5mL phosphate buffer
  • 35μL R6G in 1.5mL phosphate buffer

3) Take UV-Vis spectra of stock solutions and supernatants

Part 2: Observing pH changes

1) Separate crystal violet containing hydrogels into two epi-test tubes

2) Add 1mL of 1M HCl to one vial and 1mL NaOH to the other

3) Centrifuge for 2 min at 11200rpm to concentrate hydrogels at the bottom

4) Take UV-Vis of supernatants

Part 3: Prepare hydrogels with indicators

1) Measure out 3 0.5mg samples of PVOH hydrogels containing no lecithin and no dye

2) Dissolve each in 1.5mL phosphate buffer.

3) Allow time for them to separate

4) Add

Data

  • Add data and results here...




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