User:Allison K. Alix/Notebook/CHEM-581/2013/03/29

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==Procedures==
==Procedures==
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Part 1: preparing concentration gradient for Uv_Vis measurements
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Part 1: Preparing Concentration Gradients for UV-Vis Measurements
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1) centrifuge 6 solutions containing varying amounts of R6G prepared on [[User:Allison K. Alix/Notebook/CHEM-581/2013/03/27|03/27/2013]] for 5 minutes at 11200rpm 3 times.
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1) Centrifuge 6 solutions containing varying amounts of R6G prepared on [[User:Allison K. Alix/Notebook/CHEM-581/2013/03/27|03/27/2013]] for 5 minutes at 11200rpm 3 times.
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2) Measure the absorbance of all 3 supernatants as well as a stock solution.
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2) Make the following stock solutions to compare the supernatant absorbances to:
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*10μL R6G in 1.5mL phosphate buffer
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*15μL R6G in 1.5mL phosphate buffer
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*20μL R6G in 1.5mL phosphate buffer
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*25μL R6G in 1.5mL phosphate buffer
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*30μL R6G in 1.5mL phosphate buffer
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*35μL R6G in 1.5mL phosphate buffer
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3) Take UV-Vis spectra of stock solutions and supernatants
==Data==
==Data==

Revision as of 13:21, 29 March 2013

Experimental Chemistry Main project page
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Objective

-Centrifuge concentration gradient solutions to observe absorbance in each

-Observe pH changes in crystal violet hydrogels with the addition of HCl or NaOH

Procedures

Part 1: Preparing Concentration Gradients for UV-Vis Measurements

1) Centrifuge 6 solutions containing varying amounts of R6G prepared on 03/27/2013 for 5 minutes at 11200rpm 3 times.

2) Make the following stock solutions to compare the supernatant absorbances to:

  • 10μL R6G in 1.5mL phosphate buffer
  • 15μL R6G in 1.5mL phosphate buffer
  • 20μL R6G in 1.5mL phosphate buffer
  • 25μL R6G in 1.5mL phosphate buffer
  • 30μL R6G in 1.5mL phosphate buffer
  • 35μL R6G in 1.5mL phosphate buffer

3) Take UV-Vis spectra of stock solutions and supernatants

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


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