User:Allison K. Alix/Notebook/CHEM-581/2013/03/29: Difference between revisions
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==Procedures== | ==Procedures== | ||
Part 1: | Part 1: Preparing Concentration Gradients for UV-Vis Measurements | ||
1) | 1) Centrifuge 6 solutions containing varying amounts of R6G prepared on [[User:Allison K. Alix/Notebook/CHEM-581/2013/03/27|03/27/2013]] for 5 minutes at 11200rpm 3 times. | ||
2) | 2) Make the following stock solutions to compare the supernatant absorbances to: | ||
*10μL R6G in 1.5mL phosphate buffer | |||
*15μL R6G in 1.5mL phosphate buffer | |||
*20μL R6G in 1.5mL phosphate buffer | |||
*25μL R6G in 1.5mL phosphate buffer | |||
*30μL R6G in 1.5mL phosphate buffer | |||
*35μL R6G in 1.5mL phosphate buffer | |||
3) Take UV-Vis spectra of stock solutions and supernatants | |||
==Data== | ==Data== |
Revision as of 10:21, 29 March 2013
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Objective-Centrifuge concentration gradient solutions to observe absorbance in each -Observe pH changes in crystal violet hydrogels with the addition of HCl or NaOH ProceduresPart 1: Preparing Concentration Gradients for UV-Vis Measurements 1) Centrifuge 6 solutions containing varying amounts of R6G prepared on 03/27/2013 for 5 minutes at 11200rpm 3 times. 2) Make the following stock solutions to compare the supernatant absorbances to:
3) Take UV-Vis spectra of stock solutions and supernatants Data
NotesThis area is for any observations or conclusions that you would like to note.
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