User:Allison K. Alix/Notebook/CHEM-581/2013/03/29: Difference between revisions
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==Objective== | ==Objective== | ||
== | -Centrifuge concentration gradient solutions to observe absorbance in each | ||
-Observe pH changes in crystal violet hydrogels with the addition of HCl or NaOH | |||
==Procedures== | |||
Part 1: Preparing Concentration Gradients for UV-Vis Measurements | |||
1) Centrifuge 6 solutions containing varying amounts of R6G prepared on [[User:Allison K. Alix/Notebook/CHEM-581/2013/03/27|03/27/2013]] for 5 minutes at 11200rpm 3 times. | |||
2) Make the following stock solutions to compare the supernatant absorbances to: | |||
*10μL R6G in 1.5mL phosphate buffer | |||
*15μL R6G in 1.5mL phosphate buffer | |||
*20μL R6G in 1.5mL phosphate buffer | |||
*25μL R6G in 1.5mL phosphate buffer | |||
*30μL R6G in 1.5mL phosphate buffer | |||
*35μL R6G in 1.5mL phosphate buffer | |||
3) Take UV-Vis spectra of stock solutions and supernatants | |||
Part 2: Observing pH changes | |||
1) Separate crystal violet containing hydrogels into two epi-test tubes | |||
2) Add 1mL of 1M HCl to one vial and 1mL NaOH to the other | |||
3) Centrifuge for 2 min at 11200rpm to concentrate hydrogels at the bottom | |||
4) Take UV-Vis of supernatants | |||
Part 3: Prepare hydrogels with indicators | |||
1) Measure out 3 0.5mg samples of PVOH hydrogels containing no lecithin and no dye | |||
2) Dissolve each in 1.5mL phosphate buffer. | |||
3) Allow time for them to separate | |||
4) Add | |||
==Data== | ==Data== | ||
* Add data and results here... | * Add data and results here... | ||
Revision as of 05:08, 3 April 2013
 Experimental Chemistry
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Objective-Centrifuge concentration gradient solutions to observe absorbance in each -Observe pH changes in crystal violet hydrogels with the addition of HCl or NaOH ProceduresPart 1: Preparing Concentration Gradients for UV-Vis Measurements 1) Centrifuge 6 solutions containing varying amounts of R6G prepared on 03/27/2013 for 5 minutes at 11200rpm 3 times. 2) Make the following stock solutions to compare the supernatant absorbances to:
3) Take UV-Vis spectra of stock solutions and supernatants Part 2: Observing pH changes 1) Separate crystal violet containing hydrogels into two epi-test tubes 2) Add 1mL of 1M HCl to one vial and 1mL NaOH to the other 3) Centrifuge for 2 min at 11200rpm to concentrate hydrogels at the bottom 4) Take UV-Vis of supernatants Part 3: Prepare hydrogels with indicators 1) Measure out 3 0.5mg samples of PVOH hydrogels containing no lecithin and no dye 2) Dissolve each in 1.5mL phosphate buffer. 3) Allow time for them to separate 4) Add Data
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