User:Allison K. Alix/Notebook/CHEM-581/2013/03/29: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Procedures== | ==Procedures== | ||
Part 1: | Part 1: Preparing Concentration Gradients for UV-Vis Measurements | ||
1) | 1) Centrifuge 6 solutions containing varying amounts of R6G prepared on [[User:Allison K. Alix/Notebook/CHEM-581/2013/03/27|03/27/2013]] for 5 minutes at 11200rpm 3 times. | ||
2) Make the following stock solutions to compare the supernatant absorbances to: | |||
*10μL R6G in 1.5mL phosphate buffer | |||
*15μL R6G in 1.5mL phosphate buffer | |||
*20μL R6G in 1.5mL phosphate buffer | |||
*25μL R6G in 1.5mL phosphate buffer | |||
*30μL R6G in 1.5mL phosphate buffer | |||
*35μL R6G in 1.5mL phosphate buffer | |||
3) Take UV-Vis spectra of stock solutions and supernatants | |||
Part 2: Observing pH changes | |||
1) Separate crystal violet containing hydrogels into two epi-test tubes | |||
2) Add 1mL of 1M HCl to one vial and 1mL NaOH to the other | |||
3) Centrifuge for 2 min at 11200rpm to concentrate hydrogels at the bottom | |||
4) Take UV-Vis of supernatants | |||
Part 3: Prepare hydrogels with indicators | |||
1) Measure out 3 0.5mg samples of PVOH hydrogels containing no lecithin and no dye | |||
2) Dissolve each in 1.5mL phosphate buffer. | |||
3) Allow time for them to separate | |||
4) Add | |||
==Data== | ==Data== | ||
* Add data and results here... | * Add data and results here... | ||
Latest revision as of 22:35, 26 September 2017
Experimental Chemistry | Main project page Previous entry Next entry |
Objective-Centrifuge concentration gradient solutions to observe absorbance in each -Observe pH changes in crystal violet hydrogels with the addition of HCl or NaOH ProceduresPart 1: Preparing Concentration Gradients for UV-Vis Measurements 1) Centrifuge 6 solutions containing varying amounts of R6G prepared on 03/27/2013 for 5 minutes at 11200rpm 3 times. 2) Make the following stock solutions to compare the supernatant absorbances to:
3) Take UV-Vis spectra of stock solutions and supernatants Part 2: Observing pH changes 1) Separate crystal violet containing hydrogels into two epi-test tubes 2) Add 1mL of 1M HCl to one vial and 1mL NaOH to the other 3) Centrifuge for 2 min at 11200rpm to concentrate hydrogels at the bottom 4) Take UV-Vis of supernatants Part 3: Prepare hydrogels with indicators 1) Measure out 3 0.5mg samples of PVOH hydrogels containing no lecithin and no dye 2) Dissolve each in 1.5mL phosphate buffer. 3) Allow time for them to separate 4) Add Data
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