User:Allison K. Alix/Notebook/CHEM-581/2013/03/29: Difference between revisions

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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span>
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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Part 3: Prepare hydrogels with indicators
Part 3: Prepare hydrogels with indicators


1) Measure out 3 0.5mg samples of PVOh hydrogels containing no lecithin and no dye
1) Measure out 3 0.5mg samples of PVOH hydrogels containing no lecithin and no dye


2) Dissolve each in 1.5mL phosphate buffer.
2) Dissolve each in 1.5mL phosphate buffer.

Latest revision as of 22:35, 26 September 2017

Experimental Chemistry Main project page
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Objective

-Centrifuge concentration gradient solutions to observe absorbance in each

-Observe pH changes in crystal violet hydrogels with the addition of HCl or NaOH

Procedures

Part 1: Preparing Concentration Gradients for UV-Vis Measurements

1) Centrifuge 6 solutions containing varying amounts of R6G prepared on 03/27/2013 for 5 minutes at 11200rpm 3 times.

2) Make the following stock solutions to compare the supernatant absorbances to:

  • 10μL R6G in 1.5mL phosphate buffer
  • 15μL R6G in 1.5mL phosphate buffer
  • 20μL R6G in 1.5mL phosphate buffer
  • 25μL R6G in 1.5mL phosphate buffer
  • 30μL R6G in 1.5mL phosphate buffer
  • 35μL R6G in 1.5mL phosphate buffer

3) Take UV-Vis spectra of stock solutions and supernatants

Part 2: Observing pH changes

1) Separate crystal violet containing hydrogels into two epi-test tubes

2) Add 1mL of 1M HCl to one vial and 1mL NaOH to the other

3) Centrifuge for 2 min at 11200rpm to concentrate hydrogels at the bottom

4) Take UV-Vis of supernatants

Part 3: Prepare hydrogels with indicators

1) Measure out 3 0.5mg samples of PVOH hydrogels containing no lecithin and no dye

2) Dissolve each in 1.5mL phosphate buffer.

3) Allow time for them to separate

4) Add

Data

  • Add data and results here...




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