User:Allison K. Alix/Notebook/CHEM-581/2013/04/03: Difference between revisions
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1) Add 50μL of phenolphthalein to 5mg hydrogel in 1.5mL buffer prepared on [[User:Allison K. Alix/Notebook/CHEM-581/2013/03/29|03/29/2013]] | 1) Add 50μL of phenolphthalein to 5mg hydrogel in 1.5mL buffer prepared on [[User:Allison K. Alix/Notebook/CHEM-581/2013/03/29|03/29/2013]] | ||
2) Add 25μL bromocresol purple to 5mg hydrogels in 1.5mL phosphate buffer | |||
3) Add 75μL 0.02% cresol red to 5mg hydrogels in 1.5mL phosphate buffer | |||
4) Allow time for hydrogels to absorb indicator | |||
Part 2: Create concentration gradient for fluroescence calibration of microscope | |||
1) Prepare a solution of 10μL FluoSpheres carboxylate modified microspheres, (0.1μM, orange fluorescent (540/560) 2% solids) in 100μL distilled water (solution 1) 0.2% | |||
2) Prepare the following subsequent dilutions | |||
*solution 2: 10μL solution 1 in 100μL distilled water 0.02% | |||
*solution 3: 10μL solution 2 in 100μL distilled water 0.002% | |||
*solution 4: 10μL solution 3 in 100μL distilled water 0.0002% | |||
==Data== | ==Data== |
Revision as of 06:07, 3 April 2013
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Objective-Mix hydrogels with remaining indicators (bromocresol purple, phenolphthalein, cresol red) DescriptionPart 1: Addition of indicators to hydrogel solutions 1) Add 50μL of phenolphthalein to 5mg hydrogel in 1.5mL buffer prepared on 03/29/2013 2) Add 25μL bromocresol purple to 5mg hydrogels in 1.5mL phosphate buffer 3) Add 75μL 0.02% cresol red to 5mg hydrogels in 1.5mL phosphate buffer 4) Allow time for hydrogels to absorb indicator Part 2: Create concentration gradient for fluroescence calibration of microscope 1) Prepare a solution of 10μL FluoSpheres carboxylate modified microspheres, (0.1μM, orange fluorescent (540/560) 2% solids) in 100μL distilled water (solution 1) 0.2% 2) Prepare the following subsequent dilutions
Data
NotesThis area is for any observations or conclusions that you would like to note.
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