User:Allison K. Alix/Notebook/CHEM-581/2013/04/03

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1) Add 50μL of phenolphthalein to 5mg hydrogel in 1.5mL buffer prepared on [[User:Allison K. Alix/Notebook/CHEM-581/2013/03/29|03/29/2013]]
1) Add 50μL of phenolphthalein to 5mg hydrogel in 1.5mL buffer prepared on [[User:Allison K. Alix/Notebook/CHEM-581/2013/03/29|03/29/2013]]
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10micL in 100micL
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2) Add 25μL bromocresol purple to 5mg hydrogels in 1.5mL phosphate buffer
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3) Add 75μL 0.02% cresol red to 5mg hydrogels in 1.5mL phosphate buffer
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4) Allow time for hydrogels to absorb indicator
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Part 2: Create concentration gradient for fluroescence calibration of microscope
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1) Prepare a solution of 10μL FluoSpheres carboxylate modified microspheres, (0.1μM, orange fluorescent (540/560) 2% solids) in 100μL distilled water (solution 1) 0.2%
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2) Prepare the following subsequent dilutions
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*solution 2: 10μL solution 1 in 100μL distilled water 0.02%
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*solution 3: 10μL solution 2 in 100μL distilled water 0.002%
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*solution 4: 10μL solution 3 in 100μL distilled water 0.0002%
==Data==
==Data==

Revision as of 08:07, 3 April 2013

Experimental Chemistry Main project page
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Objective

-Mix hydrogels with remaining indicators (bromocresol purple, phenolphthalein, cresol red)

Description

Part 1: Addition of indicators to hydrogel solutions

1) Add 50μL of phenolphthalein to 5mg hydrogel in 1.5mL buffer prepared on 03/29/2013

2) Add 25μL bromocresol purple to 5mg hydrogels in 1.5mL phosphate buffer

3) Add 75μL 0.02% cresol red to 5mg hydrogels in 1.5mL phosphate buffer

4) Allow time for hydrogels to absorb indicator

Part 2: Create concentration gradient for fluroescence calibration of microscope

1) Prepare a solution of 10μL FluoSpheres carboxylate modified microspheres, (0.1μM, orange fluorescent (540/560) 2% solids) in 100μL distilled water (solution 1) 0.2%

2) Prepare the following subsequent dilutions

  • solution 2: 10μL solution 1 in 100μL distilled water 0.02%
  • solution 3: 10μL solution 2 in 100μL distilled water 0.002%
  • solution 4: 10μL solution 3 in 100μL distilled water 0.0002%

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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