User:Allison K. Alix/Notebook/CHEM-581/2013/04/03
Experimental Chemistry | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Objective-Mix hydrogels with remaining indicators (bromocresol purple, phenolphthalein, cresol red) -view hydrogels containing fluorescent dye (R6G) under fluorescent microscope ProceduresPart 1: Addition of indicators to hydrogel solutions 1) Add 50μL of phenolphthalein to 5mg hydrogel in 1.5mL buffer prepared on 03/29/2013 2) Add 25μL bromocresol purple to 5mg hydrogels in 1.5mL phosphate buffer 3) Add 75μL 0.02% cresol red to 5mg hydrogels in 1.5mL phosphate buffer 4) Allow time for hydrogels to absorb indicator Part 2: Create concentration gradient for fluroescence calibration of microscope 1) Prepare a solution of 10μL FluoSpheres carboxylate modified microspheres, (0.1μM, orange fluorescent (540/560) 2% solids) in 100μL distilled water (solution 1) 0.2% 2) Prepare the following subsequent dilutions
Part 3: Preparing dilutions of dye containing hydrogels (marked with *) 1) Dilute 2mg hydrogels containing 30μL R6G (supernatant was previously removed) with 400μL phosphate buffer (solution 1)
Data
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