User:Allison K. Alix/Notebook/CHEM-581/2013/04/10: Difference between revisions

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==Procedures==
==Procedures==


Part 1: Filtering hydrogels
'''Part 1: Filtering hydrogels'''


1) Pour top layer of safflower oil out of vials A1, B1, B2, C1, C2, D1, and D2
1) Pour top layer of safflower oil out of vials A1, B1, B2, C1, C2, D1, and D2
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5) Re-disperse filtered hydrogels in phosphate buffer
5) Re-disperse filtered hydrogels in phosphate buffer


Part 2: Observation of the addition of an acid/base to hydrogels containing indicators
'''Part 2: Observation of the addition of an acid/base to hydrogels containing indicators'''


==Data==
==Data==
* Add data and results here...


==Notes==
[[Image:SolutionA.png]]
This area is for any observations or conclusions that you would like to note.
 
Image 1: filtered hydrogels in phosphate buffer
 
[[Image:SolutionAaftersonication.png]]


Image 2: filtered hydrogels after ultrasonic liquid processing at 15 watts


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
==Notes==


[[Category:Course]]
*For next time. Sonicate hydrogels containing dye using ultrasonic liquid processor at 15 watts. Filter the (hopefully) milky solution first through regular filter paper. Then, take the filtrant (whatever is in the flask) and filter through nylon filter paper.
[[Category:Miscellaneous]]




Revision as of 09:06, 12 April 2013

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Objective

  • Filter hydrogels and observe under microscope
  • Observe indicator color changes with change in pH

Procedures

Part 1: Filtering hydrogels

1) Pour top layer of safflower oil out of vials A1, B1, B2, C1, C2, D1, and D2

2) Add hexanes to each vial and vortex

3) Filter each hydrogel sample using vacuum filtration

4) Combine samples B1 and B2, C1 and C2, and D1 and D2

5) Re-disperse filtered hydrogels in phosphate buffer

Part 2: Observation of the addition of an acid/base to hydrogels containing indicators

Data

Image 1: filtered hydrogels in phosphate buffer

Image 2: filtered hydrogels after ultrasonic liquid processing at 15 watts

Notes

  • For next time. Sonicate hydrogels containing dye using ultrasonic liquid processor at 15 watts. Filter the (hopefully) milky solution first through regular filter paper. Then, take the filtrant (whatever is in the flask) and filter through nylon filter paper.



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