User:Allison K. Alix/Notebook/CHEM-581/2013/04/12: Difference between revisions
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==Objectives== | ==Objectives== | ||
* Sonicate hydrogels containing dye using ultra sonic liquid processing and filter | * Sonicate hydrogels containing dye using ultra sonic liquid processing and filter (2x) | ||
* Mix sonicated hydrogels containing no dye with dye and indicators to monitor absorbance | |||
==Procedures== | ==Procedures== | ||
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2) Sonicate for ~5 minutes at 15 watts | 2) Sonicate for ~5 minutes at 15 watts | ||
3) Filter using vacuum filtration (Filter used: Quantitative P8, Porosity: Coarse, Flow Rate: Fast) | |||
4) Filter the filtrant once more through a nylon filter. | |||
5) Take absorbance of remaining filtrant | |||
6) Redisperse hydrogels on filter paper back into 10 mL phosphate buffer and observe under microscope | |||
Part 2: Mixing ultrasonicated hydrogels | |||
The following samples were prepared: | |||
* | |||
==Data== | ==Data== |
Revision as of 12:39, 12 April 2013
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Objectives
ProceduresPart 1: Sonication of Hydrogels 1) Measure ~7.5mL of solution C 2) Sonicate for ~5 minutes at 15 watts 3) Filter using vacuum filtration (Filter used: Quantitative P8, Porosity: Coarse, Flow Rate: Fast) 4) Filter the filtrant once more through a nylon filter. 5) Take absorbance of remaining filtrant 6) Redisperse hydrogels on filter paper back into 10 mL phosphate buffer and observe under microscope Part 2: Mixing ultrasonicated hydrogels The following samples were prepared: Data
NotesThis area is for any observations or conclusions that you would like to note.
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