User:Allison K. Alix/Notebook/Fluorescence Detection of Telomerase Activity/2012/11/15

From OpenWetWare

Jump to: navigation, search
(Entry title)
Current revision (15:26, 15 November 2012) (view source)
(Procedure)
 
(One intermediate revision not shown.)
Line 10: Line 10:
-take fluorescence measurements of several mixtures all of which contain cancer cell solution
-take fluorescence measurements of several mixtures all of which contain cancer cell solution
 +
 +
==Procedure==
 +
 +
Preparation of Mixtures:
 +
 +
1) a. 5μL 2mM dNTP soln, 100μL cell solution, 50μL Elaine's primer, 25μL telomerase complementary strand, 25μL Molecular Beacon
 +
  b. 5μL 2mM dNTP soln, 100μL cell solution, 50μL primer attached to AuNP, 25μL telomerase complementary strand, 25μL Molecular Beacon
 +
 +
2) a. 5μL 2mM dNTP soln, 100μL cell solution, 50μL Elaine's primer, 25μL Molecular Beacon
 +
  b. 5μL 2mM dNTP soln, 100μL cell solution, 50μL primer attached to AuNP, 25μL Molecular Beacon
 +
 +
3) 5μL 2mM dNTP soln, 100μL cell solution, 50μL Elaine's primer, 10μL 5μM Zinc Protoporphyrin IX
 +
 +
4) 5μL 2mM dNTP soln, 100μL cell solution, 50μL primer attached to AuNP, 10μL 5μM Zinc Protoporphyrin IX
 +
 +
5) a. 5μL 2mM dNTP soln, 100μL cell solution, 50μL primer attached to AuNP, 25μL telomerase complementary strand
 +
  b. b. 5μL 2mM dNTP soln, 100μL cell solution, 50μL Elaine's primer, 25μL telomerase complementary strand
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Current revision

Project name Main project page

Objectives

-take fluorescence measurements of several mixtures all of which contain cancer cell solution


Procedure

Preparation of Mixtures:

1) a. 5μL 2mM dNTP soln, 100μL cell solution, 50μL Elaine's primer, 25μL telomerase complementary strand, 25μL Molecular Beacon

  b. 5μL 2mM dNTP soln, 100μL cell solution, 50μL primer attached to AuNP, 25μL telomerase complementary strand, 25μL Molecular Beacon

2) a. 5μL 2mM dNTP soln, 100μL cell solution, 50μL Elaine's primer, 25μL Molecular Beacon

  b. 5μL 2mM dNTP soln, 100μL cell solution, 50μL primer attached to AuNP, 25μL Molecular Beacon

3) 5μL 2mM dNTP soln, 100μL cell solution, 50μL Elaine's primer, 10μL 5μM Zinc Protoporphyrin IX

4) 5μL 2mM dNTP soln, 100μL cell solution, 50μL primer attached to AuNP, 10μL 5μM Zinc Protoporphyrin IX

5) a. 5μL 2mM dNTP soln, 100μL cell solution, 50μL primer attached to AuNP, 25μL telomerase complementary strand

  b. b. 5μL 2mM dNTP soln, 100μL cell solution, 50μL Elaine's primer, 25μL telomerase complementary strand


Personal tools