User:Allison K. Alix/Notebook/Thesis Research/2013/01/15: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Fluorescence Measurements</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Fluorescence Measurements</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Re-running Fluorescence Measurements==
==Procedures==


* Fluorescence Spectra for the following samples were obtained (excited at 515nm)
Obtain fluorescence spectra for the following samples:(excited at 515nm)


* concentrated AuNP - (3x10^-9 M)
* concentrated AuNP (3x10^-9 M)
* 1:6 diluted AuNP (6.49 x10^-9 M)  
* 1:6 diluted AuNP (6.49 x10^-9 M)  
* 1:6 dilution with thiol primer attached. (10μL diluted to 100μL with 90μL water)
* 1:6 diluted AuNP with thiol primer attached. (10μL diluted to 100μL with 90μL water)
* Elaine's Primer (10μL diluted to 100μL with 90μL H<sub>2</sub>O)\
* Elaine's Thiol Primer (10μL diluted to 100μL with 90μL H<sub>2</sub>O)\
* Telomerase Complementary Strand (10μL diluted to 100μL with 90μL H<sub>2</sub>O)
* Telomerase Complementary Strand (10μL diluted to 100μL with 90μL H<sub>2</sub>O)
Telomerase Molecular Beacon was diluted with 1mL filtered H<sub>2</sub>O, separated into vials in 10μL aliquots and placed in the freezer.
==Data==
[[Image:FluorSpecAll.jpg]]


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Latest revision as of 22:21, 26 September 2017

Fluorescence Measurements Main project page
Next entry

Procedures

Obtain fluorescence spectra for the following samples:(excited at 515nm)

  • concentrated AuNP (3x10^-9 M)
  • 1:6 diluted AuNP (6.49 x10^-9 M)
  • 1:6 diluted AuNP with thiol primer attached. (10μL diluted to 100μL with 90μL water)
  • Elaine's Thiol Primer (10μL diluted to 100μL with 90μL H2O)\
  • Telomerase Complementary Strand (10μL diluted to 100μL with 90μL H2O)

Telomerase Molecular Beacon was diluted with 1mL filtered H2O, separated into vials in 10μL aliquots and placed in the freezer.

Data


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