User:Allison K. Alix/Notebook/Thesis Research/2013/01/22: Difference between revisions
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== | ==Procedure== | ||
Hybridize Molecular Beacon with the following @ same concentration in 1:1 ratios: | |||
1) Elaine's Primer | |||
2) Gold nano-particles (1:6 Dilution) | |||
3) | |||
Add 500μL H<sub>2</sub>O to DNA strands: | |||
4-stranded: | |||
132.8nmoles/0.5mL x 1000ml/1L = 265.6μM | |||
8-stranded: | |||
71.4nmoles/0.5mL x 1000mL/1L = 142.8μM | |||
1) | |||
Revision as of 10:51, 22 January 2013
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ProcedureHybridize Molecular Beacon with the following @ same concentration in 1:1 ratios: 1) Elaine's Primer 2) Gold nano-particles (1:6 Dilution) 3) Add 500μL H2O to DNA strands: 4-stranded: 132.8nmoles/0.5mL x 1000ml/1L = 265.6μM 8-stranded: 71.4nmoles/0.5mL x 1000mL/1L = 142.8μM 1)
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