User:Allison K. Alix/Notebook/Thesis Research/2013/01/22
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ProceduresHybridize Molecular Beacon (731nM) with the following @ same concentration in 1:1 ratios: 1) Elaine's Primer (307.8nM) To get 307.8nM MB: 731nM(x) = (307.8nM)(5μL) x = 2.105μL MB in 2.895μLH_{2}O mix with 5μL Elaines Primer and 180μL filtered H_{2}O
To get 6.49nM MB: 731nm(x) = (6.49nM)(100μL) x = 0.89μL MB in 99.11 μL H_{2}O mix with 100μL AuNP _____
1) 4 telomeric repeats: 132.8nmoles/0.5mL x 1000ml/1L = 265.6μM 2) 8 telomeric repeats: 71.4nmoles/0.5mL x 1000mL/1L = 142.8μM
1) 4 telomeric repeats: To get 5μM : 265.6 μM (x) = 5μM (100μL) x = 1.88 μL in 98.12μL H_{2}O mix with 100μL ZnPPIX 2) 8 telomeric repeats: To get 5μM : 142.8 μM (x) = 5μM (100μL) x = 3.5 μL in 96.5 μL H_{2}O mix with 100μL ZnPPIX Take Absorbance/Fluorescence of all 4 hybridizations
