User:Allison K. Alix/Notebook/Thesis Research/2013/01/29: Difference between revisions
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5μM ZnPPIX already prepared | 5μM ZnPPIX already prepared | ||
''To prepare 1 μM 4 telomeric repeat DNA:'' | |||
265.6μM (x) = 1μM (100μL) | 265.6μM (x) = 1μM (100μL) | ||
x = 2.656 μL in 97.344 μL H<sub>2</sub>O | x = '''2.656 μL''' in 97.344 μL H<sub>2</sub>O | ||
''To prepare 1 μM 8 telomeric repeat DNA:'' | |||
142.8μM (x) = 1μM (100μL) | 142.8μM (x) = 1μM (100μL) | ||
x = 1.428 μL in 98.572 μL H<sub>2</sub>O | x = '''1.428 μL''' in 98.572 μL H<sub>2</sub>O | ||
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Revision as of 17:04, 4 February 2013
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Objectives-Repeat ZnPPIX and DNA hybridizations in 5:1 ratios instead of 1:1 -Hybridize ThT and DNA strands Procedures5μM ZnPPIX already prepared To prepare 1 μM 4 telomeric repeat DNA: 265.6μM (x) = 1μM (100μL) x = 2.656 μL in 97.344 μL H2O To prepare 1 μM 8 telomeric repeat DNA: 142.8μM (x) = 1μM (100μL) x = 1.428 μL in 98.572 μL H2O | |
