User:Allison K. Alix/Notebook/Thesis Research/2013/01/29

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(Procedures)
(Procedures)
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1. prepare 5mM tris-HCl buffer solution
1. prepare 5mM tris-HCl buffer solution
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  121.4 g/mol x 5mmol/L x 1mol/1000mmol x 50 mL x 1L/1000mL = 0.030285 g in 50 mL
+
121.4 g/mol x 5mmol/L x 1mol/1000mmol x 50 mL x 1L/1000mL = 0.030285 g in 50 mL
-
  Add 1 N HCl (~8 drops) to obtain pH ~ 7.25
+
- Add 1 N HCl (~8 drops) to obtain pH ~ 7.25
2.  
2.  

Revision as of 20:08, 4 February 2013

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Objectives

-Repeat ZnPPIX and DNA hybridizations in 5:1 ratios instead of 1:1

-Hybridize ThT and DNA strands

Procedures

ZnPPIX:

5μM ZnPPIX already prepared

1. Prepare 1 μM 4 telomeric repeat DNA:

265.6μM (x) = 1μM (100μL)

x = 2.656 μL in 97.344 μL H2O

2. Prepare 1 μM 8 telomeric repeat DNA

142.8μM (x) = 1μM (100μL)

x = 1.428 μL in 98.572 μL H2O

ThT

1. prepare 5mM tris-HCl buffer solution

121.4 g/mol x 5mmol/L x 1mol/1000mmol x 50 mL x 1L/1000mL = 0.030285 g in 50 mL

- Add 1 N HCl (~8 drops) to obtain pH ~ 7.25

2.



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