User:Allison K. Alix/Notebook/Thesis Research/2013/01/29: Difference between revisions
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==Procedures== | ==Procedures== | ||
'''ZnPPIX:''' | |||
5μM ZnPPIX already prepared | 5μM ZnPPIX already prepared | ||
1. Prepare 1 μM 4 telomeric repeat DNA: | |||
265.6μM (x) = 1μM (100μL) | 265.6μM (x) = 1μM (100μL) | ||
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x = '''2.656 μL''' in 97.344 μL H<sub>2</sub>O | x = '''2.656 μL''' in 97.344 μL H<sub>2</sub>O | ||
2. Prepare 1 μM 8 telomeric repeat DNA | |||
142.8μM (x) = 1μM (100μL) | 142.8μM (x) = 1μM (100μL) | ||
x = '''1.428 μL''' in 98.572 μL H<sub>2</sub>O | x = '''1.428 μL''' in 98.572 μL H<sub>2</sub>O | ||
'''ThT''' | |||
1. prepare 5mM tris-HCl buffer solution | |||
121.4 g/mol x 5mmol/L x 1mol/1000mmol x 50 mL x 1L/1000mL = 0.030285 g in 50 mL | |||
Add 1 N HCl (~8 drops) to obtain pH ~ 7.25 | |||
2. | |||
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Revision as of 17:08, 4 February 2013
 Project name
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Objectives-Repeat ZnPPIX and DNA hybridizations in 5:1 ratios instead of 1:1 -Hybridize ThT and DNA strands ProceduresZnPPIX: 5μM ZnPPIX already prepared 1. Prepare 1 μM 4 telomeric repeat DNA: 265.6μM (x) = 1μM (100μL) x = 2.656 μL in 97.344 μL H2O 2. Prepare 1 μM 8 telomeric repeat DNA 142.8μM (x) = 1μM (100μL) x = 1.428 μL in 98.572 μL H2O ThT 1. prepare 5mM tris-HCl buffer solution 121.4 g/mol x 5mmol/L x 1mol/1000mmol x 50 mL x 1L/1000mL = 0.030285 g in 50 mL Add 1 N HCl (~8 drops) to obtain pH ~ 7.25 2.
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