User:Allison K. Alix/Notebook/Thesis Research/2013/02/12: Difference between revisions
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*Take Fluorescence Measurements of samples from [[User:Allison_K._Alix/Notebook/Thesis_Research/2013/02/05|2/5/2013]] | *Take Fluorescence Measurements of samples from [[User:Allison_K._Alix/Notebook/Thesis_Research/2013/02/05|2/5/2013]] | ||
* We are expecting to see a decrease in signal with decreasing DNA concentration | * We are expecting to see a decrease in signal with decreasing DNA concentration | ||
=Spectra= | |||
[[Image:4repeatDNAThTfluorgraph.jpg]] | |||
[[Image:8repeatDNAThTfluorgraph.jpg]] | |||
[[Image:Fluorintcomp1.5micM.png]] | |||
[[Image:Fluorintcomp2.0micM.png]] | |||
[[Image:Fluorintcomp2.5micM.png]] | |||
[[Image:Fluorintcomp3.0micM.png]] | |||
[[Image:Fluorintcomp3.5micM.png]] | |||
=Observations= | |||
4 repeat telomeric DNA samples: | |||
There was only a trend of increasing intensity with increasing concentration of DNA seen in the 1.5μM, 3.0μM, and 3.5μM samples. The 2.0μM and 2.5μM samples were very much out of order. Next step is to plot intensity at max peak vs concentration. | |||
[[Image:Fluorintvsconc4strandpic.png]] | |||
[[Image:Fluorintvsconc8strandpic.png]] | |||
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Revision as of 11:15, 19 February 2013
 Fluorescence Measurements
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Objectives
Spectra
Observations4 repeat telomeric DNA samples: There was only a trend of increasing intensity with increasing concentration of DNA seen in the 1.5μM, 3.0μM, and 3.5μM samples. The 2.0μM and 2.5μM samples were very much out of order. Next step is to plot intensity at max peak vs concentration.
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