User:Allison K. Alix/Notebook/Thesis Research/2013/02/19: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> ZnPPIX/DNA and ThT/DNA Sample Prep</span> | ||
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Revision as of 10:38, 19 February 2013
 ZnPPIX/DNA and ThT/DNA Sample Prep
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ObjectivesPrepare samples with 5μM ZnPPIX and varying concentrations of 4-strand and 8-strand telomeric DNA Remake 2.0μM and 2.5μM 4-strand DNA and 2.5μM 8-strand DNA to be mixed with 3.5μM ThT (these need to be remade as they were outliers in our previous fluorescence measurements from 02/12/2013 Procedures1) Prepare the following concentrations of DNA using calculations from 02/05/2013. Use tris-HCL buffer to dilute.
2) Mix each of the DNA samples with 250μL 5μM ZnPPIX 3) Also prepare an extra set of 2.0μM and 2.5μM 4-strand telomerics DNA and 2.5μM 8-strand DNA 4) Mix those DNA samples with 250μL 3.5μM ThT 5) Heat all samples (total of 13) above 70°C to hybridize for 25 minutes 6) Obtain UV-Vis and Fluorescence Spectra of all samples
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