User:Allison K. Alix/Notebook/Thesis Research/2013/03/29

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Objectives

-Perform Gel Electrophoresis on DNA-AuNP solution as well as supernatants

-Perform FCS on ThT/DNA-AuNP and MB/DNA-AuNP

Procedures

Part 1:

1) Dissolve 0.35g agarose in a tupperware container with 35mL 1X TAE buffer

  • Prepare 50mL 1X buffer from 50X buffer
  • 50X(V)=(1X)(50mL)
  • V= 1mL 50X in 49mL H2O

2) Microwave for ~10 seconds at a time for ~1min to dissolve

3) Cool slightly (AKA make sure it isn't boiling and you are able to touch the container safely)

4) Pour into casting tray and allow to solidify (20 to 30 minutes). Make sure the two end pieces and comb are in place.

5) Once solidified, remove the comb, tilting as you pull it out.

6) Prepare the DNA samples including the DNA Ladder

  • Combine 4μL of the following samples with 2μL loading buffer

a) 100bp DNA Ladder

b) DNA-AuNP Solution

c) Supernatant 1

d) Supernatant 2

e) Supernatant 3




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