# User:Allison K. Alix/Notebook/Thesis Research/2013/04/02

(Difference between revisions)
 Revision as of 11:57, 2 April 2013 (view source) (→Procedures)← Previous diff Revision as of 12:04, 2 April 2013 (view source) (→Procedures)Next diff → Line 26: Line 26: x= 13.7μL in 486.3μL H2O x= 13.7μL in 486.3μL H2O - 1455nm(x) = + 1455nM(x) = 727.5nm(200μL) + + x= 100μL 1455nM in 100μL H2O + + 2) Prepare 800nm MB + + (Original concentration of MB is 7.31μM) + + 7130nm(x) = (800nM)(50μL) + + x = 5.47μL 7.31μM in 44.53 μL H2O + + 3) Mix 50μL 727.5nM DNA with 50μL 800nM MB + + 4) Hybridize by placing mixture on heat block @ 76°C for ~25minutes + + 5) Turn off heat block and allow cooling to room temperature + +

## Revision as of 12:04, 2 April 2013

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## Objectives

• Bind DNA to MB and AuNP
• React AuNP with ethanethiol

## Procedures

Part 1: Reacting DNA with MB first, followed by a reaction of the DNA-MB hybrid with AuNP

1) Prepare 727.5nm 8-repeat DNA-thiol and 800nm MB

• 727.5nm 8-repeat DNA-thiol

(Original concentration of DNA is 53.1μM)

53.1μM(x)=(500μL)(1455nm)

x= 13.7μL in 486.3μL H2O

1455nM(x) = 727.5nm(200μL)

x= 100μL 1455nM in 100μL H2O

2) Prepare 800nm MB

(Original concentration of MB is 7.31μM)

7130nm(x) = (800nM)(50μL)

x = 5.47μL 7.31μM in 44.53 μL H2O

3) Mix 50μL 727.5nM DNA with 50μL 800nM MB

4) Hybridize by placing mixture on heat block @ 76°C for ~25minutes

5) Turn off heat block and allow cooling to room temperature