# User:Allison K. Alix/Notebook/Thesis Research/2013/04/02

(Difference between revisions)
 Revision as of 12:05, 2 April 2013 (view source) (→Procedures)← Previous diff Revision as of 12:08, 2 April 2013 (view source) (→Procedures)Next diff → Line 18: Line 18: 1) Prepare 727.5nm 8-repeat DNA-thiol and 800nm MB 1) Prepare 727.5nm 8-repeat DNA-thiol and 800nm MB - * 727.5nm 8-repeat DNA-thiol + a) 727.5nm 8-repeat DNA-thiol (Original concentration of DNA is 53.1μM) (Original concentration of DNA is 53.1μM) Line 30: Line 30: x= 100μL 1455nM in 100μL H2O x= 100μL 1455nM in 100μL H2O - 2) Prepare 800nm MB + b) Prepare 800nm MB (Original concentration of MB is 7.31μM) (Original concentration of MB is 7.31μM) Line 43: Line 43: 5) Turn off heat block and allow cooling to room temperature 5) Turn off heat block and allow cooling to room temperature + + '''Part 2: Reacting DNA with ThT first, followed by a reaction of the DNA-ThT hybrid with AuNP''' + + 1) Prepare 727.5nM DNA (see Part 1, step 1a) + + 2) Prepare 800nM ThT + + *Note that the ThT prepared on [[Link title]]

## Revision as of 12:08, 2 April 2013

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## Objectives

• Bind DNA to MB and AuNP
• React AuNP with ethanethiol

## Procedures

Part 1: Reacting DNA with MB first, followed by a reaction of the DNA-MB hybrid with AuNP

1) Prepare 727.5nm 8-repeat DNA-thiol and 800nm MB

a) 727.5nm 8-repeat DNA-thiol

(Original concentration of DNA is 53.1μM)

53.1μM(x)=(500μL)(1455nm)

x= 13.7μL in 486.3μL H2O

1455nM(x) = 727.5nm(200μL)

x= 100μL 1455nM in 100μL H2O

b) Prepare 800nm MB

(Original concentration of MB is 7.31μM)

7130nm(x) = (800nM)(50μL)

x = 5.47μL 7.31μM in 44.53 μL H2O

3) Mix 50μL 727.5nM DNA with 50μL 800nM MB

4) Hybridize by placing mixture on heat block @ 76°C for ~25minutes

5) Turn off heat block and allow cooling to room temperature

Part 2: Reacting DNA with ThT first, followed by a reaction of the DNA-ThT hybrid with AuNP

1) Prepare 727.5nM DNA (see Part 1, step 1a)

2) Prepare 800nM ThT