User:Allison K. Alix/Notebook/Thesis Research/2013/04/02

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Procedures)
(Procedures)
Line 18: Line 18:
1) Prepare 727.5nm 8-repeat DNA-thiol and 800nm MB
1) Prepare 727.5nm 8-repeat DNA-thiol and 800nm MB
-
* 727.5nm 8-repeat DNA-thiol
+
a) 727.5nm 8-repeat DNA-thiol
(Original concentration of DNA is 53.1μM)
(Original concentration of DNA is 53.1μM)
Line 30: Line 30:
x= 100μL 1455nM in 100μL H<sub>2</sub>O
x= 100μL 1455nM in 100μL H<sub>2</sub>O
-
2) Prepare 800nm MB
+
b) Prepare 800nm MB
(Original concentration of MB is 7.31μM)
(Original concentration of MB is 7.31μM)
Line 43: Line 43:
5) Turn off heat block and allow cooling to room temperature
5) Turn off heat block and allow cooling to room temperature
 +
 +
'''Part 2: Reacting DNA with ThT first, followed by a reaction of the DNA-ThT hybrid with AuNP'''
 +
 +
1) Prepare 727.5nM DNA (see Part 1, step 1a)
 +
 +
2) Prepare 800nM ThT
 +
 +
*Note that the ThT prepared on [[Link title]]

Revision as of 11:08, 2 April 2013

Project name Main project page
Previous entry      Next entry

Objectives

  • Bind DNA to MB and AuNP
  • React AuNP with ethanethiol

Procedures

Part 1: Reacting DNA with MB first, followed by a reaction of the DNA-MB hybrid with AuNP

1) Prepare 727.5nm 8-repeat DNA-thiol and 800nm MB

a) 727.5nm 8-repeat DNA-thiol

(Original concentration of DNA is 53.1μM)

53.1μM(x)=(500μL)(1455nm)

x= 13.7μL in 486.3μL H2O

1455nM(x) = 727.5nm(200μL)

x= 100μL 1455nM in 100μL H2O

b) Prepare 800nm MB

(Original concentration of MB is 7.31μM)

7130nm(x) = (800nM)(50μL)

x = 5.47μL 7.31μM in 44.53 μL H2O

3) Mix 50μL 727.5nM DNA with 50μL 800nM MB

4) Hybridize by placing mixture on heat block @ 76°C for ~25minutes

5) Turn off heat block and allow cooling to room temperature

Part 2: Reacting DNA with ThT first, followed by a reaction of the DNA-ThT hybrid with AuNP

1) Prepare 727.5nM DNA (see Part 1, step 1a)

2) Prepare 800nM ThT






Personal tools