User:Allison K. Alix/Notebook/Thesis Research/2013/04/02: Difference between revisions
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3) Mix 50μL 727.5nM DNA with 50μL 800nM ThT | 3) Mix 50μL 727.5nM DNA with 50μL 800nM ThT | ||
Part 3: React AuNP's with ethanethiol | |||
1) Calculate how many sulfur atoms to cover 1 nanoparticle | |||
* Diameter of AuNP (synthesized 3/1/13 by Dr. Miller) is 14nm, so radius, r=7nm | |||
* A = 4π(r<sup>2</sup>) | |||
A = 4π(7nm<sup>2</sup>) | |||
A = 615.75nm<sup>2</sup> | |||
Revision as of 09:37, 2 April 2013
 Project name
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Objectives
ProceduresPart 1: Reacting DNA with MB first, followed by a reaction of the DNA-MB hybrid with AuNP 1) Prepare 727.5nm 8-repeat DNA-thiol and 800nm MB a) 727.5nm 8-repeat DNA-thiol (Original concentration of DNA is 53.1μM) 53.1μM(x)=(500μL)(1455nm) x= 13.7μL in 486.3μL H2O 1455nM(x) = 727.5nm(200μL) x= 100μL 1455nM in 100μL H2O b) Prepare 800nm MB (Original concentration of MB is 7.31μM) 7130nm(x) = (800nM)(50μL) x = 5.47μL 7.31μM in 44.53 μL H2O 3) Mix 50μL 727.5nM DNA with 50μL 800nM MB 4) Hybridize by placing mixture on heat block @ 76°C for ~25minutes 5) Turn off heat block and allow cooling to room temperature Part 2: Reacting DNA with ThT first, followed by a reaction of the DNA-ThT hybrid with AuNP 1) Prepare 727.5nM DNA (see Part 1, step 1a) 2) Prepare 800nM ThT
5mM(x) = (5μM)(10mL) 10μL of 5mM ThT in 9990μL buffer 5μM (x) = (800nm)(1mL) x= 160μL 5μM ThT in 840μL buffer 3) Mix 50μL 727.5nM DNA with 50μL 800nM ThT Part 3: React AuNP's with ethanethiol 1) Calculate how many sulfur atoms to cover 1 nanoparticle
A = 4π(7nm2) A = 615.75nm2
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