User:Allison K. Alix/Notebook/Thesis Research/2013/04/04: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Abs and Fluor Measurements</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Procedures== | ==Procedures== | ||
Part 1: Measure absorbance values | Part 1: Measure absorbance values of hybrid | ||
thiol-DNA/ThT | |||
fluorescence: | |||
1) excitation at 400. Start 410, end 610 (according to absorbance spec | |||
2) excitation at 440 start at 450 end 650 | |||
3) excitation at 417 start at 427 end 650 | |||
Part 2: Measure fluorescence of ThT and DNA alone | |||
==Observations== | |||
* The concentration of ThT used in this solution was likely too low. Although ThT was in excess, the absorbance seen on the UV-Vis spectrum was very low. It is interesting to note that the DNA-ThT hybrid did show slight fluorescence intensity. Because there is a possibility that this signal was a background signal coming from the ThT, a fluorescence spectrum of that same concentration of ThT alone in solution was also taken (See Data below) | |||
==Data== | |||
Absorbance: | |||
[[Image:ThiolDNAThThybridUVVisspec.png]] | |||
Fluorescence: | |||
[[Image:ThiolDNAThThybridFluorescencespec.png]] | |||
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Latest revision as of 22:36, 26 September 2017
Abs and Fluor Measurements | Main project page Previous entry Next entry |
Objectives
ProceduresPart 1: Measure absorbance values of hybrid thiol-DNA/ThT fluorescence: 1) excitation at 400. Start 410, end 610 (according to absorbance spec 2) excitation at 440 start at 450 end 650 3) excitation at 417 start at 427 end 650 Part 2: Measure fluorescence of ThT and DNA alone Observations
DataAbsorbance: Fluorescence: |