User:Allison K. Alix/Notebook/Thesis Research/2013/04/04

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(Procedures)
(Procedures)
Line 15: Line 15:
Part 1: Measure absorbance values
Part 1: Measure absorbance values
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thiol-DNA/ThT
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fluorescence:
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1) excitation at 400. Start 410, end 610 (according to absorbance spec
 +
 +
2) excitation at 440 start at 450 end 650
 +
 +
3) excitation at 417 start at 427 end 650

Revision as of 13:09, 4 April 2013

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Objectives

  • Measure absorbance and fluorescence of thiol-DNA/MB and thiol-DNA/ThT mixtures prepared on 04/02/2013
  • React both with AuNP

Procedures

Part 1: Measure absorbance values

thiol-DNA/ThT

fluorescence:

1) excitation at 400. Start 410, end 610 (according to absorbance spec

2) excitation at 440 start at 450 end 650

3) excitation at 417 start at 427 end 650



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