User:Allison K. Alix/Notebook/Thesis Research/2013/04/04: Difference between revisions

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Part 1: Measure absorbance values
Part 1: Measure absorbance values
thiol-DNA/ThT
fluorescence:
1) excitation at 400. Start 410, end 610 (according to absorbance spec
2) excitation at 440 start at 450 end 650
3) excitation at 417 start at 427 end 650





Revision as of 11:09, 4 April 2013

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Objectives

  • Measure absorbance and fluorescence of thiol-DNA/MB and thiol-DNA/ThT mixtures prepared on 04/02/2013
  • React both with AuNP

Procedures

Part 1: Measure absorbance values

thiol-DNA/ThT

fluorescence:

1) excitation at 400. Start 410, end 610 (according to absorbance spec

2) excitation at 440 start at 450 end 650

3) excitation at 417 start at 427 end 650