User:Allison K. Alix/Notebook/Thesis Research/2013/04/05

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(Autocreate 2013/04/05 Entry for User:Allison_K._Alix/Notebook/Thesis_Research)
Current revision (10:19, 12 April 2013) (view source)
 
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Reacting thiol-DNA with ThT</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
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==Objectives==
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* Insert content here...
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* Mix DNA with higher concentration of ThT
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* Measure absorbance and fluorescence of DNA/ThT hybrid
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==Procedures==
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Part 1: Mixing thiol-DNA with ThT
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1) Mix 200μL of 5μM ThT with 200μL 1.455μM thiol-DNA
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* Note that new concentrations of reactants in solution are 2.5μM ThT and 0.7275μM DNA
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2) Place on heat block at ~76°C for 25 minutes
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3) Allow solution to cool on heat block until at room temperature
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4) Take absorbance and fluorescence measurements
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* AuNP will then be added to this solution
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Part 2: Mixing thiol-DNA with MB
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Prepare MB (need at least 100μL)
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12μL (7.31μM) = x(100μL)
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x = 0.8772μM = 877.2nM
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mix 100μL with 100μL 727.5nM DNA

Current revision

Reacting thiol-DNA with ThT Main project page
Previous entry      Next entry

Objectives

  • Mix DNA with higher concentration of ThT
  • Measure absorbance and fluorescence of DNA/ThT hybrid

Procedures

Part 1: Mixing thiol-DNA with ThT

1) Mix 200μL of 5μM ThT with 200μL 1.455μM thiol-DNA

  • Note that new concentrations of reactants in solution are 2.5μM ThT and 0.7275μM DNA

2) Place on heat block at ~76°C for 25 minutes

3) Allow solution to cool on heat block until at room temperature

4) Take absorbance and fluorescence measurements

  • AuNP will then be added to this solution

Part 2: Mixing thiol-DNA with MB

Prepare MB (need at least 100μL)

12μL (7.31μM) = x(100μL)

x = 0.8772μM = 877.2nM

mix 100μL with 100μL 727.5nM DNA



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