User:Allison K. Alix/Notebook/Thesis Research/2013/04/05: Difference between revisions
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== | ==Objectives== | ||
* | * Mix DNA with higher concentration of ThT | ||
* Measure absorbance and fluorescence of DNA/ThT hybrid | |||
==Procedures== | |||
Part 1: Mixing thiol-DNA with ThT | |||
1) Mix 200μL of 5μM ThT with 200μL 1.455μM thiol-DNA | |||
* Note that new concentrations of reactants in solution are 2.5μM ThT and 0.7275μM DNA | |||
2) Place on heat block at ~76°C for 25 minutes | |||
3) Allow solution to cool on heat block until at room temperature | |||
4) Take absorbance and fluorescence measurements | |||
* AuNP will then be added to this solution | |||
Part 2: Mixing thiol-DNA with MB | |||
Prepare MB (need at least 100μL) | |||
12μL (7.31μM) = x(100μL) | |||
x = 0.8772μM = 877.2nM | |||
mix 100μL with 100μL 727.5nM DNA | |||
Revision as of 07:38, 5 April 2013
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Objectives
ProceduresPart 1: Mixing thiol-DNA with ThT 1) Mix 200μL of 5μM ThT with 200μL 1.455μM thiol-DNA
2) Place on heat block at ~76°C for 25 minutes 3) Allow solution to cool on heat block until at room temperature 4) Take absorbance and fluorescence measurements
Part 2: Mixing thiol-DNA with MB Prepare MB (need at least 100μL) 12μL (7.31μM) = x(100μL) x = 0.8772μM = 877.2nM mix 100μL with 100μL 727.5nM DNA
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