User:Allison K. Alix/Notebook/Thesis Research/2013/04/05: Difference between revisions
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==Procedures== | ==Procedures== | ||
Part 1: Mixing thiol-DNA with ThT | |||
1) Mix 200μL of 5μM ThT with 200μL 1.455μM thiol-DNA | |||
* Note that new concentrations of reactants in solution are 2.5μM ThT and 0.7275μM DNA | |||
2) Place on heat block at ~76°C for 25 minutes | |||
3) Allow solution to cool on heat block until at room temperature | |||
4) Take absorbance and fluorescence measurements | |||
* AuNP will then be added to this solution | |||
Revision as of 07:00, 5 April 2013
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Objectives
ProceduresPart 1: Mixing thiol-DNA with ThT 1) Mix 200μL of 5μM ThT with 200μL 1.455μM thiol-DNA
2) Place on heat block at ~76°C for 25 minutes 3) Allow solution to cool on heat block until at room temperature 4) Take absorbance and fluorescence measurements
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