User:Allison K. Alix/Notebook/Thesis Research/2013/04/05: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Reacting thiol-DNA with ThT</span> | ||
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Revision as of 08:19, 12 April 2013
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Objectives
ProceduresPart 1: Mixing thiol-DNA with ThT 1) Mix 200μL of 5μM ThT with 200μL 1.455μM thiol-DNA
2) Place on heat block at ~76°C for 25 minutes 3) Allow solution to cool on heat block until at room temperature 4) Take absorbance and fluorescence measurements
Part 2: Mixing thiol-DNA with MB Prepare MB (need at least 100μL) 12μL (7.31μM) = x(100μL) x = 0.8772μM = 877.2nM mix 100μL with 100μL 727.5nM DNA
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