User:Allison K. Alix/Notebook/Thesis Research/2013/04/05: Difference between revisions

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==Procedures==
==Procedures==
Part 1: Mixing thiol-DNA with ThT
1) Mix 200μL of 5μM ThT with 200μL 1.455μM thiol-DNA
* Note that new concentrations of reactants in solution are 2.5μM ThT and 0.7275μM DNA
2) Place on heat block at ~76°C for 25 minutes
3) Allow solution to cool on heat block until at room temperature
4) Take absorbance and fluorescence measurements
* AuNP will then be added to this solution





Revision as of 07:00, 5 April 2013

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Objectives

  • Mix DNA with higher concentration of ThT
  • Measure absorbance and fluorescence of DNA/ThT hybrid

Procedures

Part 1: Mixing thiol-DNA with ThT

1) Mix 200μL of 5μM ThT with 200μL 1.455μM thiol-DNA

  • Note that new concentrations of reactants in solution are 2.5μM ThT and 0.7275μM DNA

2) Place on heat block at ~76°C for 25 minutes

3) Allow solution to cool on heat block until at room temperature

4) Take absorbance and fluorescence measurements

  • AuNP will then be added to this solution