User:Allison K. Alix/Notebook/Thesis Research/2013/04/09: Difference between revisions

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{|{{table}} width="800"
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Reacting Ethane thiol with AuNP</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==Procedures==
==Procedures==


Part 1: Preparation of 1mL of 2.98μM ethane thiol
'''Part 1: Preparation of 1mL of 2.98μM ethane thiol'''


* original concentrationof AuNP = 19.4nM
WARNING: ethanethiol is very volatile. Handle under the hood only wearing PPE.
 
* original concentration of AuNP = 19.4nM
* desired concentration of ethanethiol = 19.4nM x(15393) = 298624.2nM = 298μM
* desired concentration of ethanethiol = 19.4nM x(15393) = 298624.2nM = 298μM
* density of ethanethiol = 0.839g/mL
* density of ethanethiol = 0.839g/mL


1) Calculating concentration of ethanethiol
1) Find the concentration of 0.0125mL pure ethanethiol in 5mL water
 
0.839g/mL x 0.0125mL = 0.0104875g
 
0.0104875g/(62.13 g/mol) = 1.68799 x 10<sup>-4</sup> mol


0.839g/mL x 0.025mL = 0.020975g
1.68799 x 10<sup>-4</sup> mol/5mL x (1000mL/1L) = 0.034M = 34mM


0.020975g/(62.13 g/mol) = 3.376 x 10<sup>-4</sup> mol


3.376 x 10<sup>-4</sup> mol/1mL x (1000mL/1L) = 0.34M = 340mM


340mM (x) = (30mM)(1mL)
340mM (x) = (30mM)(1mL)
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* react 200μL 298μM ethane thiol with 200μL 19.4nM AuNP (synthesized by Dr. Miller)
* react 200μL 298μM ethane thiol with 200μL 19.4nM AuNP (synthesized by Dr. Miller)
'''Part 2: Measure absorbance/fluorescence of AuNP-MB'''
'''Part 3: Measure absorbance/fluorescence of AuNP-ThT'''
==Data==
Part 2:
[[Image:ThiolDNA_MB_pic_UV-Vis.png‎]]
UV-Vis Spectrum of thiol-DNA/MB hybrid
[[Image:ThiolDNA_MB_pic.png]]
Fluorescence Spectrum of thiol-DNA/MB hybrid
Part 3:
[[Image:ThiolDNA_ThT_pic_UV-Vis.png‎]]
UV-Vis Spectrum of thiol-DNA/ThT hybrid
[[Image:ThiolDNA ThT pic.png]]
Fluorescence Spectrum of thiol-DNA/ThT hybrid




Latest revision as of 22:37, 26 September 2017

Reacting Ethane thiol with AuNP Main project page
Previous entry      Next entry

Objectives

  • Take Abs and Fluorescence measurements of DNA-MB and DNA-ThT prepared on 4/4/13
  • React ethane-thiol with AuNP

Procedures

Part 1: Preparation of 1mL of 2.98μM ethane thiol

WARNING: ethanethiol is very volatile. Handle under the hood only wearing PPE.

  • original concentration of AuNP = 19.4nM
  • desired concentration of ethanethiol = 19.4nM x(15393) = 298624.2nM = 298μM
  • density of ethanethiol = 0.839g/mL

1) Find the concentration of 0.0125mL pure ethanethiol in 5mL water

0.839g/mL x 0.0125mL = 0.0104875g

0.0104875g/(62.13 g/mol) = 1.68799 x 10-4 mol

1.68799 x 10-4 mol/5mL x (1000mL/1L) = 0.034M = 34mM


340mM (x) = (30mM)(1mL)

x = 0.088mL = 88μL in 912μL H2O

30mM (x) = 3mM (1mL)

x = 100μL in 900μL

3mM(x) = 298μM (1mL)

x = 99μL in 901μL to obtain 298μM ethanethiol

2) Reacting AuNP with ethanethiol

  • react 200μL 298μM ethane thiol with 200μL 19.4nM AuNP (synthesized by Dr. Miller)

Part 2: Measure absorbance/fluorescence of AuNP-MB

Part 3: Measure absorbance/fluorescence of AuNP-ThT

Data

Part 2:

UV-Vis Spectrum of thiol-DNA/MB hybrid

Fluorescence Spectrum of thiol-DNA/MB hybrid

Part 3:

UV-Vis Spectrum of thiol-DNA/ThT hybrid

Fluorescence Spectrum of thiol-DNA/ThT hybrid



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