User:Allison K. Alix/Notebook/Thesis Research/2013/05/14: Difference between revisions
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* NEW concentrations: 8.9μM thiol-DNA and 10μM ThT | * NEW concentrations: 8.9μM thiol-DNA and 10μM ThT | ||
2) | 2) Heat at 75°C for ~25min | ||
3) Cool to room temperature | |||
4) Take UV-Vis/Fluorescence measurements of thiol-DNA/ThT sample | |||
'''Part 2: Hybridizing thiol-DNA/ThT with AuNP''' | |||
1) | |||
Revision as of 07:55, 14 May 2013
 Project name
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Objectives
Procedures
Previous concentrations used:
To be used:
Calculations: Preparing 17.8μM thiol-DNA original concentration of thiol-DNA- 53.1μM 53.1μM (x) = (17.8μM)(500μL) x= 167.63μL in 332.37μL buffer Preparing 20μM ThT original conc ThT= 5mM 5mM (x) = (0.02mM)(10mL) x=40μL in 9960μL H2O
1) Mix 250μL 17.8μM thiol-DNA with 250μL 20μM ThT
2) Heat at 75°C for ~25min 3) Cool to room temperature 4) Take UV-Vis/Fluorescence measurements of thiol-DNA/ThT sample Part 2: Hybridizing thiol-DNA/ThT with AuNP 1)
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