User:Allison K. Alix/Notebook/Thesis Research/2013/05/23: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Observe Fluor w/ and w/o AuNP</span> | ||
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Revision as of 12:38, 3 June 2013
 Observe Fluor w/ and w/o AuNP
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Objectives
ProceduresPart 1: Adding ThT to thiol-DNA/AuNP [ThT] must be twice what [DNA] is [DNA] = 1.11225μM, so [ThT] must be 2.2245μM Use 250μL of DNA-AuNP solution (10%=25μM) 2.2245x10-6M x 275x10-6L solution = 6.117375x10-10 moles ThT (6.117375x10-10 moles ThT)/ 25 x 10-6 L = 24.4695μM -Add 25μL of 24.4695μM ThT to 250μL thiol-DNA/AuNP solution NEW Concentrations: [DNA]= 1.011μM [AuNP] = 13.486μM [ThT] = 2.2245μM Take Fluorescence Spectrum Part 2: Preparing thiol-DNA/ThT solution using same concentrations in thiol-DNA/AuNP/ThT solution 1) Prepare 250μL of 2.022μM thiol DNA (5.82μM)(x) = (250μL)(2.022μM) x = 86.85 μL 5.82μM thiol-DNA in 163.15μL PBS Buffer 2) Prepare 250μL of 4.449μM ThT (5μM)(x) = (4.449μM)(250μL) x = 222.45μL in 27.55μL PBS Buffer 3) Mix 250μL 2.002μM thiol-DNA with 250μL 4.449μM ThT 4) Obtain Fluorescence Spectrum Data
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