User:Allison K. Alix/Notebook/Thesis Research/2013/05/29: Difference between revisions

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==Procedures==
==Procedures==
Part 1: Preparation of 100mM DTT in 4% TEA solution
Original DTT concentration 1M
1M(x) = (100x10<sup>-3</sup>M)(1mL)
x = 0.1mL = 100μL in 900μL 4% TEA
4%(900μL) = 36μL TEA in 864μL PBS
OVERALL: 100μL 1M DTT + 36μL TEA + 864μL PBS




Revision as of 07:26, 29 May 2013

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Objectives

  • Prepare DTT/TEA solution to be used in DNA extraction procedures.

Procedures

Part 1: Preparation of 100mM DTT in 4% TEA solution

Original DTT concentration 1M

1M(x) = (100x10-3M)(1mL)

x = 0.1mL = 100μL in 900μL 4% TEA

4%(900μL) = 36μL TEA in 864μL PBS

OVERALL: 100μL 1M DTT + 36μL TEA + 864μL PBS




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