User:Allison K. Alix/Notebook/Thesis Research/2013/05/30
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Abs/Fluor of MB Samples | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Objectives
ProceduresPart 1: Preparing thiol-DNA and MB 1) Prepare 727.5nM thiol DNA (2910nM) (x) = (727.5nM)(100μL) x = 25μL in 75μL PBS 2) Prepare 1.268μM MB (7.61μM)(20μL) = x(120) x = 1.286μM Dilute 20μL MB in 100μL DNA to obtain a concentration of 1.286μM 3) Heat @ ~75°C for 25 minutes Dataconcentration of DNA= 677.157nM |