User:Allison K. Alix/Notebook/Thesis Research/2013/05/31: Difference between revisions

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==Procedures==
==Procedures==
Part 1: Preparation of AuNP-DNA sample
Final concentrations of AuNP and DNA should be 14.835nM and 1.11625μM respectively
1) 53.1μM(x)=(4.4506μM)(250μL)
x= 20.95μL in 229.05μL PBS
mix 20.95μL 53.1μM thiol-DNa with 229.05μL PBS
2) Mix 250μL DNA solution with 250μL 100mM DTT in 4% TEA
NEW [DNA] = 2.225μM
3) React 10 minutes
5) Extract DTT from solution using 4 2mL aliquots of ethyl acetate
6) Mix 232.4μL DNA, 232.4μL 29.7nM AuNP, and 36.2μL sodium citrate buffer
NEW [DNA] = 1.034μM
NEW {[AuNP] = 13.805nM
7) React 10 minutes




Revision as of 12:08, 3 June 2013

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Objectives

  • Prepare an AuNP/DNA sample
  • Prepare ThT/DNA sample
  • Prepare MB sample
  • Prepare ThT sample
  • Prepare 0.05M phosphate buffer

Procedures

Part 1: Preparation of AuNP-DNA sample

Final concentrations of AuNP and DNA should be 14.835nM and 1.11625μM respectively

1) 53.1μM(x)=(4.4506μM)(250μL)

x= 20.95μL in 229.05μL PBS

mix 20.95μL 53.1μM thiol-DNa with 229.05μL PBS

2) Mix 250μL DNA solution with 250μL 100mM DTT in 4% TEA

NEW [DNA] = 2.225μM

3) React 10 minutes

5) Extract DTT from solution using 4 2mL aliquots of ethyl acetate

6) Mix 232.4μL DNA, 232.4μL 29.7nM AuNP, and 36.2μL sodium citrate buffer

NEW [DNA] = 1.034μM NEW {[AuNP] = 13.805nM

7) React 10 minutes



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