User:Allison K. Alix/Notebook/Thesis Research/2013/06/05: Difference between revisions
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Zinc protoporphyrin (ZnPPIX) samples will be prepared at the same concentrations, following the same procedure as the ThT samples. | Zinc protoporphyrin (ZnPPIX) samples will be prepared at the same concentrations, following the same procedure as the ThT samples. | ||
2.22μM ZnPPIX and 1.11265μM DNA | 2.22μM ZnPPIX and 1.11265μM DNA | ||
(5μM)V=(4.44μM)(300μL) | (5μM)V=(4.44μM)(300μL) | ||
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V=12.57μL DNA in 287.43μL | V=12.57μL DNA in 287.43μL | ||
ZnPPIX, DNA and AuNP | ZnPPIX, DNA and AuNP | ||
2[ThT]:1[DNA]→ 2.068μM ZnPPIX | 2[ThT]:1[DNA]→ 2.068μM ZnPPIX | ||
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(5.31476×10-10moles)/(25×10-6L)=21.3μM ZnPPIX | (5.31476×10-10moles)/(25×10-6L)=21.3μM ZnPPIX | ||
Make 21.3μM ZnPPIX: | Make 21.3μM ZnPPIX: | ||
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V=0.107μL in 24.9μL | V=0.107μL in 24.9μL | ||
Add 25μL of 21.3μM ZnPPIX to 250μL DNA/AuNP solution | Add 25μL of 21.3μM ZnPPIX to 250μL DNA/AuNP solution | ||
Concentrations: | Concentrations: |
Revision as of 08:52, 5 June 2013
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Objectives
ProceduresPart 1: Preparing solutions for coating AuNP with Silica 1) dissolve 0.716g PVP (10kg/mol) in 10mL water (0.00716M) 2) Dissolve 0.42mL NH4OH (29.3wt% NH3 in H2O) in 9.58mL Ethanol (200 proof) (4.2vol%) NOTE: TES solution will be prepared immediately prior to use to minimize exposure Part 2: Preparing ZnPPIX solutions Zinc protoporphyrin (ZnPPIX) samples will be prepared at the same concentrations, following the same procedure as the ThT samples.
V=266.4μL 5μM ZnPPIX in 33.6μL (53.1μ)V=(2.2253μM)(300μL) V=12.57μL DNA in 287.43μL
10% of 250μL=25μL (within 10% allows for accurate concentration comparison) Total solution volume: 275μL (2.068×10-6M)(275×10-6L)=5.31476×10-10moles ZnPPIX (5.31476×10-10moles)/(25×10-6L)=21.3μM ZnPPIX
(5000μM)V=(21.3μM)(25μL) V=0.107μL in 24.9μL
DNA- 0.94μM AuNP- 12.55nM ZnPPIX- 1.9364μM 2.22μM ZnPPIX (5μM)V=(2.22μM)(300μL) V=133.2μL ZnPPIX in 166.8μL
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