User:Allison K. Alix/Notebook/Thesis Research/2013/06/05

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(Procedures)
(Procedures)
Line 24: Line 24:
Zinc protoporphyrin (ZnPPIX) samples will be prepared at the same concentrations, following the same procedure as the ThT samples.
Zinc protoporphyrin (ZnPPIX) samples will be prepared at the same concentrations, following the same procedure as the ThT samples.
 +
2.22μM ZnPPIX and 1.11265μM DNA
2.22μM ZnPPIX and 1.11265μM DNA
 +
(5μM)V=(4.44μM)(300μL)
(5μM)V=(4.44μM)(300μL)
Line 34: Line 36:
V=12.57μL DNA in 287.43μL
V=12.57μL DNA in 287.43μL
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ZnPPIX, DNA and AuNP
ZnPPIX, DNA and AuNP
 +
2[ThT]:1[DNA]→ 2.068μM ZnPPIX
2[ThT]:1[DNA]→ 2.068μM ZnPPIX
Line 46: Line 50:
(5.31476×10-10moles)/(25×10-6L)=21.3μM ZnPPIX
(5.31476×10-10moles)/(25×10-6L)=21.3μM ZnPPIX
 +
Make 21.3μM ZnPPIX:
Make 21.3μM ZnPPIX:
Line 52: Line 57:
V=0.107μL in 24.9μL
V=0.107μL in 24.9μL
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Add 25μL of 21.3μM ZnPPIX to 250μL DNA/AuNP solution
Add 25μL of 21.3μM ZnPPIX to 250μL DNA/AuNP solution
 +
Concentrations:
Concentrations:

Revision as of 11:52, 5 June 2013

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Objectives

Procedures

Part 1: Preparing solutions for coating AuNP with Silica

1) dissolve 0.716g PVP (10kg/mol) in 10mL water (0.00716M)

2) Dissolve 0.42mL NH4OH (29.3wt% NH3 in H2O) in 9.58mL Ethanol (200 proof) (4.2vol%)

NOTE: TES solution will be prepared immediately prior to use to minimize exposure

Part 2: Preparing ZnPPIX solutions

Zinc protoporphyrin (ZnPPIX) samples will be prepared at the same concentrations, following the same procedure as the ThT samples.


2.22μM ZnPPIX and 1.11265μM DNA


(5μM)V=(4.44μM)(300μL)

V=266.4μL 5μM ZnPPIX in 33.6μL

(53.1μ)V=(2.2253μM)(300μL)

V=12.57μL DNA in 287.43μL


ZnPPIX, DNA and AuNP


2[ThT]:1[DNA]→ 2.068μM ZnPPIX

10% of 250μL=25μL (within 10% allows for accurate concentration comparison)

Total solution volume: 275μL

(2.068×10-6M)(275×10-6L)=5.31476×10-10moles ZnPPIX

(5.31476×10-10moles)/(25×10-6L)=21.3μM ZnPPIX


Make 21.3μM ZnPPIX:

(5000μM)V=(21.3μM)(25μL)

V=0.107μL in 24.9μL


Add 25μL of 21.3μM ZnPPIX to 250μL DNA/AuNP solution


Concentrations:

DNA- 0.94μM

AuNP- 12.55nM

ZnPPIX- 1.9364μM

2.22μM ZnPPIX

(5μM)V=(2.22μM)(300μL)

V=133.2μL ZnPPIX in 166.8μL



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