User:Allison K. Alix/Notebook/Thesis Research/2013/06/12
From OpenWetWare
(→Calculating AuNP concentration in g/L) 
(→Calculating AuNP concentration in g/L) 

Line 55:  Line 55:  
4) Calculate mass of all AuNP in 20mL solution  4) Calculate mass of all AuNP in 20mL solution  
  5.7668x10<sup>16</sup> g/1 AuNP x 5.219x10<sup>12</sup>AuNP x 1/20mL x 1000mL/1L = 0.1505g/L  +  5.7668x10<sup>16</sup> g/1 AuNP x 5.219x10<sup>12</sup>AuNP x 1/20mL x 1000mL/1L = '''0.1505g/L''' 
Revision as of 14:01, 12 June 2013
Project name  Main project page Previous entry Next entry 
AuNP synthesis
Note: These AuNPs will be used in the silica coating procedure. See calculations below. Calculating AuNP diameter and concentrationλ_{spr}= 527nm d= [ln(λ_{spr}λ_{0}/L_{1})]/L_{2} where λ_{0}= 512nm, L_{1}= 6.53, and L_{2}=0.0216 (These are fit parameters from theoretical calculations^{1}) d= [ln(527nm512nm/6.53)]/0.0216 d= 38.5nm c = A_{450}/ε_{450} c = 1.89/(4.36x10^{9}) c = 0.43nM Calculating AuNP concentration in g/L1) Calculate the volume of 1 AuNP Volume of 1 AuNP = 4/3πr^{3}, where r = d/2 = 19.25nm V= 4/3π(19.25x10^{9}m)^{3} V= 2.988x10^{23}m^{3} V= 2.988x10^{17}cm^{3} 2) Calculate the mass of 1 AuNP density of Au = 19.3g/cm^{3} 19.3g/cm^{3} x 2.988x10^{17}cm^{3} = 5.7668x10^{16} g per 1 AuNP 3) Calculate # of AuNP in 20mL solution 0.43x10^{9}mol/L x 20mL x 1L/1000mL x 6.02x10^{23}AuNP/1 mol = 5.219x10^{12} AuNP 4) Calculate mass of all AuNP in 20mL solution 5.7668x10^{16} g/1 AuNP x 5.219x10^{12}AuNP x 1/20mL x 1000mL/1L = 0.1505g/L
